VALIDATION
In finite long-term health hazard prediction technology gains its relevance through calibration and validation procedures.
While most of the material-specific part of a digital twin is directly determined from in vitro monitoring of the cellular clusters exposed to particular material, the calibration process is used to define the material-agnostic part of a digital twin using animal response data associated with the selected set of materials, covering the widest possible range of early Mode-of-Actions with the least number of different materials.
The entire process of digital twin creation is then tested within the validation, for which a complementary set of the materials is used, deriving the prediction accuracy and precision.
IN VIVO DATASETS ASSOCIATED WITH INFLAMMATION
Entire process was run under umbrella of the Horizon Europe NanoPASS project, started in January 2023.
Employing the largest repository of datasets for the inflammatory response in mouse lungs, in particular the mice neutrophils influx in mouse lungs related to instillation exposures to 22 different materials at various doses measured at various time points up to 9 months after exposure. The data from more than 1695 mice exposures has been used from that repository.
IN FINITE DATASETS ASSOCIATED WITH INFLAMMATION
On the other hand, validation relied on 1,160 in vitro monitoring experiments delivering 1,700 early response evolutions acquired in roughly 670,000 live images.
QUALITY OF VALIDATION
In 2025, the 3rd generation of validation was delivered in Q1, employing an improved translation model with a better description of in vitro experiments. This leads to improved coverage of early Mode-of-Actions, guaranteeing applicability to a wide range of materials and high quality of long-term predictions, as shown in the figure below.
VALIDATION
In 2024, Infinite and its partners have run the first cross-species double blind validation with translated exposure patterns that provided a clear comparison of in vivo neutrophils influx data (crosses) obtained by exposing rats with different exposure schemes to predicted neutrophil influx (curves) obtained by in vitro monitoring (mice cells), translated into MoA and finally predicted into responses for different exposure schemes according to the customer cases.
In 2025, Infinite and its partners will finish also other double blind in vivo validation studies of various real industrial materials (or better material cocktails), already sampled at working places in construction material production, electronics waste management, 3D printing and dental fillings production. These studies are performed within EU NanoPASS project.
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